The technical facilities aim at suppporting the DEEP Labex research program by means of state-of-the-art technology. Their mission : to decipher the complexity of the establishment, maintenance and regulation of biological processes during development, in the germline or in adult stem cells. Studying the alteration of these pathways in pathological contexts could lead to the identification of the mechanisms involved as well as keys to interfere with the disease.
Cell biology, development and epigenesis are among those fields that require state-of-the-art microscopy and imaging technologies together with quantitative analysis techniques. In this context, the development of high resolution imaging techniques for in vivo analysis (confocal microscopy, strcutured illumination micriscopy), photomanipulation tools (two-photon microscopy and adaptative optics) and associated analysis softwares are key features of the DEEP Labex research program.
Yeast genetics has always been a quality tool to study and understand gene functions. Recent technological progress enabled the development of high speed systems that aim at using yeast genetics for functional genomic studies. The SGA (Synthetic Genomic Array) facility was set up at the Curie Institute thanks to the support of the DEEP Labex and offers one of the most utter systems for functional genomics.
Biological material is often a limiting factor when studying transcriptional and epigenomic regulations during development. Moreover, the understanding of developmental processes requires to take into account a spatiotemporal dimension. Therefore, comprehensie analysis requires to fit in several developmental stages in different contexts, wild type, mutant and pathological. The Nanostring technology performs a quantitative « meso-analysis » of multiple targets (DNA, RNA) in multiple samples simultaneaously, allowing the quantification of up to 800 targets together with a simple and immediate data treatment. It is thereby an attractive intermediate between quantitative PCR (simple analysis but restrcited to a few targets) and high throughput sequencing (total coverage but heavy bioinformatic analysis). The integration of this new tool within the genomic facility reinforces the service activity of this facility for all Curie Institute research departments as well as other institutes.