Publications

Cellules souches et potentiel Clonal analysis of Notch1-expressing cells reveals the existence of unipotent stem cells that retain long-term plasticity in the embryonic mammary gland

Lilja, A., Rodilla, V., Huyghe, M., Hannezo, E., Landragin, C., Renaud, O., Leroy, O., Ruland, S., Simons, B.D, and Fre, S. Nat Cell Biol. May 21, 2018. doi:10.1038/s41556-018-0108-1 En savoir plus Replier

Recent lineage tracing studies have revealed that mammary gland homeostasis relies on unipotent stem cells. However, whether and when lineage restriction occurs during embryonic mammary development, and which signals orchestrate cell fate specification, remain unknown. Using a combination of in vivo clonal analysis with whole mount immunofluorescence and mathematical modelling of clonal dynamics, we found that embryonic multipotent mammary cells become lineage-restricted surprisingly early in development, with evidence for unipotency as early as E12.5 and no statistically discernable bipotency after E15.5. To gain insights into the mechanisms governing the switch from multipotency to unipotency, we used gain-of-function Notch1 mice and demonstrated that Notch activation cell autonomously dictates luminal cell fate specification to both embryonic and basally committed mammary cells. These functional studies have important implications for understanding the signals underlying cell plasticity and serve to clarify how reactivation of embryonic programs in adult cells can lead to cancer.

News and Views:  Mammary lineage restriction in development. Philip Bland & Beatrice A. Howard. https://www.nature.com/articles/s41556-018-0111-6

 


Epigénétique Genetic and epigenetic features direct differential efficiency of Xist-mediated silencing at X-chromosomal and autosomal locations.

Loda A, Brandsma JH, Vassilev I, Servant N, Loos F, Amirnasr A, Splinter E, Barillot E, Poot RA, Heard E, Gribnau J. Nat Commun. 2017 Sep 25;8(1):690. (2017)

Développement et Epigenèse RNA FISH to Study Zygotic Genome Activation in Early Mouse Embryos.

Ranisavljevic N, Okamoto I, Heard E, Ancelin K. Methods Mol Biol. 2017;1605:133-145. (2017)

Développement et Epigenèse Epigénétique Cellules souches et potentiel Xist-dependent imprinted X inactivation and the early developmental consequences of its failure.

Borensztein M, Syx L, Ancelin K, Diabangouaya P, Picard C, Liu T, Liang JB, Vassilev I, Galupa R, Servant N, Barillot E, Surani A, Chen CJ, Heard E. Nat Struct Mol Biol. 2017 Mar;24(3):226-233. (2017)

Développement et Epigenèse Epigénétique Cellules souches et potentiel Landscape of monoallelic DNA accessibility in mouse embryonic stem cells and neural progenitor cells.

Xu J, Carter AC, Gendrel AV, Attia M, Loftus J, Greenleaf WJ, Tibshirani R, Heard E, Chang HY. Nat Genet. 2017 Mar;49(3):377-386. (2017)

Développement et Epigenèse Epigénétique Cellules souches et potentiel XACT Noncoding RNA Competes with XIST in the Control of X Chromosome Activity during Human Early Development.

Vallot C, Patrat C, Collier AJ, Huret C, Casanova M, Liyakat Ali TM, Tosolini M, Frydman N, Heard E, Rugg-Gunn PJ, Rougeulle C. Cell Stem Cell. 2017 Jan 5;20(1):102-111. (2017)

Epigénétique X chromosome inactivation: new players in the initiation of gene silencing.

Pinheiro I, Heard E. F1000Res. 2017 Mar 27;6. pii: F1000 Faculty Rev-344. (2017)

Epigénétique Novel players in X inactivation: insights into Xist-mediated gene silencing and chromosome conformation.

da Rocha ST, Heard E. Nat Struct Mol Biol. 2017 Mar 3;24(3):197-204. (2017)

Closing the loop: 3C versus DNA FISH.

Giorgetti L, Heard E. Genome Biol. 2016 Oct 19;17(1):215. (2017)

Epigénétique Random monoallelic expression of genes on autosomes: Parallels with X-chromosome inactivation.

Gendrel AV, Marion-Poll L, Katoh K, Heard E. Semin Cell Dev Biol. 2016 Aug;56:100-110. (2017)

Structural and functional diversity of Topologically Associating Domains.

Dekker J, Heard E. FEBS Lett. 2015 Oct 7;589(20 Pt A):2877-84. (2017)

Cellules souches et potentiel Tissue homeostasis and aging: new insight from the fly intestine.

Gervais L, Bardin AJ. Curr Opin Cell Biol. 2017 Oct;48:97-105. (2017)

Cellules souches et potentiel Intrinsic regulation of enteroendocrine fate by Numb.

Sallé J, Gervais L, Boumard B, Stefanutti M, Siudeja K, Bardin AJ. EMBO J. 2017 May 22. (2017)

Somatic recombination in adult tissues: What is there to learn?

Siudeja K, Bardin AJ. Fly (Austin). 2017 Apr 3;11(2):121-128. (2017)

Développement et Epigenèse Biomechanics of cell rearrangements in Drosophila.

Guirao B, Bellaïche Y. Curr Opin Cell Biol. 2017 Oct;48:113-124. (2017)
Archives