Offres d’emploi

Ingénieur d’Étude (IE) en biologie moléculaire et biochimie

Institut Curie, Centre de Recherche – Référence poste : « IE PIC3i-D » – 2016/11
Poste d’Ingénieur d’Étude (IE) de 18 mois en biologie moléculaire et biochimie – Institut Curie

L’Institut Curie est un organisme de renommée internationale pour la recherche contre le cancer, constitué d’un centre de recherche fondamentale et d’un hôpital pour la recherche clinique. Il est situé au coeur de Paris dans le quartier latin, au sein d’un environnement académique stimulant et interdisciplinaire.
L’unité de Physico-chimie (UMR168) et l’unité de Dynamique Nucléaire (UMR3664) recherchent une/un ingénieur(e) d’étude en biologie moléculaire et biochimie. La personne recrutée travaillera sur le développement d’une nouvelle méthode d’imagerie de sites génomiques dans des cellules eucaryotes vivantes, basée sur la technologie CRISPR/Cas9.
Missions :
-Primaire : Design/optimisation, synthèse in vitro, assemblage et purification de protéines recombinantes CRISPR/Cas9 modifiées et d’ARNs guides.
-Secondaire : Participation aux aspects d’imagerie du projet avec d’autres membres de nos équipes, spécialisés en techniques de microscopie.
-Secondaire : Extraction d’ARNs de cellules mammifères et préparation de librairies de séquençage.

Profil et compétences requises :
‐ diplôme universitaire (licence, master) en biologie moléculaire et/ou biochimie,
-­ clonage moléculaire et techniques d’ADN recombinant,
– transcription in vitro (T7, SP6) et manipulation d’échantillons d’ARN,
­‐ expression et purification de protéines recombinantes,
‐ bon niveau d’anglais oral et écrit,
­‐ autonomie et intérêt pour le travail interdisciplinaire.

Compétences complémentaires appréciées :
­‐ expérience en microscopie à fluorescence,
­‐ culture de cellules mammifères adhérentes,
­‐ extraction d’ARN, RT-qPCR, préparation de librairies de séquençage,
‐ design de vecteurs d’expression mammifère,
‐ technologies CRISPR/Cas9,
­‐ bioinformatique.
L’ingénieur(e) travaillera entre les équipes de Maxime Dahan et d’Antoine Coulon,
combinant leur savoir-faire en imagerie du noyau et microscopie de molécules
uniques et leur expertise sur l’organisation dynamique du génome eucaryote.
Type de contrat : CDD d’ingénieur d’étude à plein temps.
Salaire : en fonction de l’expérience et de la qualification.
Lieu : Institut Curie, campus principal (Paris 5ème).
Début du contrat : Dès que possible – Durée : 18 mois.
Merci d’adresser votre dossier de candidature (CV, lettre de motivation et nom/email
de 2-3 référents) à recruitment@coulonlab.org, en indiquant “Candidature IE PIC3i-D”
dans l’objet du mail. Les candidatures seront évaluées au fur et à mesure qu’elles
seront reçues, et ce jusqu’au 1er décembre 2016.

VALORIZATION MANAGER POSITION

Valorization Manager position for Labex DEEP and Labex Celtisphybio programs, Institut Curie, Paris

The Institut Curie (http://www.curie.fr) is a world-class multidisciplinary cancer research center combining research in biology, genetics, soft matter physics, organic and medicinal chemistry.

The activities of the Labex valorization manager will cover both DEEP and CelTisPhyBio, two Labex programs of the Institut Curie.

Missions

  • Industrial Valorization (80%):

o Identification of projects within the Labex teams for future industrial partnerships and patents

o Landscaping, scouting and due-diligence of industrial partners

o Liaise with the Valorization & Industrial Partnership Department for drafting of contracts, patents and “declarations d’invention”, as well as for the negotiation of agreements with industrial partners

o Initiate scientific collaborations with relevant industrial partners

o Ensure smooth running of the partnerships by coordinating scientific, administrative, financial, human and logistics aspects

o Writing of project progress reports (tasks, milestones, budget), including mid-term assessment, task reports, annual reports and final reports

Help coordinating with the labex manager and animating scientific events (seminars, courses, workshops, retreats) aimed to promote the industrial valorization of the Labex

  • Communication (20%):

o Coordinate communication within DEEP and CelTisPhyBio and with partners /associates in the PSL IDEX and more broadly in the French community and Europe

o Update content of both DEEP and CelTisPhyBio Websites

Skills

 

  • M2 or PhD in Biology or Biophysics
  • Experience of project management in the industry or with industrial partners
  • The candidate should be adaptable, proactive, able to work on several projects in parallel and have strong communication and interpersonal skills
  • Fluent english required

 

Contract : 3 year contract (COD). Salary depending on experience and qualifications.

Starting Date : January/February 2016

 

Please send your CV, cover letter and contact information for two references to recrutement.labex.deep@curie.fr

 

Application deadline : January 8th 2016

ENGINEER IN FLUORESCENCE MICROSCOPY AND IMAGE ANALYSIS

The Genetics and Development Biology Unit (http://www.ugbdd.curie.fr) of the Institut Curie is a multidisciplinary department whose main objective is the study of events controlling cell dynamics during development. The research teams intensively use optical fluorescence microscopy to visualize in real time different biological processes in various animal models (Drosophila, zebrafish and mouse).

In the context of the Labex-DEEP program (www.labex-deep.fr), we are looking to recruit an engineer in fluorescence microscopy with expertise in advanced image analysis to join the imaging facility.

 

Missions

  • Working in close collaboration with researchers on scientific projects that require intensive use of optical microscopes (e.g. super-resolution).
  • Designing and implementing tools for image analysis and processing using ImageJ / Fiji and/or Matlab.
  • Analysis of multidimensional microscopy datasets acquired by researchers.
  • Train and help scientists in microscopy and in the use of image analysis softwares.
  • Participate in the animation of the imaging facility.

 

Skills

  • University degree (Master, PhD), engineering school or equivalent degree in biology, biophysics with a specialization in image processing.
  • Having thorough knowledge of optical microscopy methods, image analysis and processing applied to biology.
  • Proven experience in the development of tools to process and analyse multidimensional fluorescence microscopy datasets using ImageJ / Fiji (macros and/or java plugin) and/or Matlab.
  • Experience in super-resolution microscopy (SIM) would be a plus.
  • Ability to work in teams and have an interest in multidisciplinary approaches in developmental biology.
  • Ability to manage multiple projects simultaneously. A high degree of autonomy is required.
  • Able to communicate in English.

 

Please send your CV, cover letter and contact information for two references to: recrutement.labex.deep@curie.fr

Contract: COD (defined time contract) for 12 months (renewable to 36 months). Salary depending on experience and qualifications.

Applications will be reviewed when they are received.

Poste AI/IE en Biologie Moléculaire

Affectation : Laboratoire de Développement de la lignée germinale Unité de Génétique et Biologie du Développement (UMR3215), Institut Curie, PARIS
Contexte : L’Institut Curie, situé au coeur de Paris, est l’un des plus importants centres européens de recherche contre le cancer. Il se compose d’un hôpital spécialisé dans le traitement des cancers, un centre de recherche fondamentale et un département de recherche translationnelle. Le poste est à pourvoir au sein de département de recherche fondamentale UMR 3215 – Unité Génétique et Biologie du Développement. Cette unité, basée sur le campus parisien de l’Institut Curie, compte 10 équipes pour 120 personnes environ. Le poste à pourvoir se situe dans le laboratoire du Dr Jean-René Huynh, qui travaille sur le développement de la lignée germinale, principalement chez la drosophile.

Mission : La personne recrutée aura pour mission d’accompagner les chercheurs du laboratoire dans leurs projets et de prendre en charge de manière autonome la partie de biologie moléculaire de chaque projet : clonage, création de plasmides, purification d’ADN, d’ARN, en vue d’obtenir des vecteurs de transgénèse pour la drosophile. Les projets concernés font appel aux techniques classiques de biologie moléculaire, ainsi qu’à des techniques plus récentes telles que CRISPR/Cas9 pour la génération de drosophiles mutantes ou exprimant un tag inséré au locus endogène. Il sera aussi possible d’utiliser des cellules mammifères en culture.

Compétences :

Savoirs généraux ou théoriques:

– Connaissances approfondies des techniques modernes de biologie moléculaire.

– Connaissances en biologie cellulaire.

– Connaissances de base en informatique (traitement et gestion des données).

Savoir-faire opérationnels

– Maîtriser les techniques de biologie moléculaire (telles que clonage, mutagénèse, PCR, RT-PCR, qRT-PCR, hybridation in situ).

– Maîtriser les techniques et protocoles de culture cellulaire (si possible avoir une expérience en transduction virale (notamment lentivirale).

– Une expérience préalable des techniques d’édition de génome est souhaitable.

– Capacité à interagir avec des interlocuteurs variés.

– Capacité à travailler en équipe dans un environnement international et en constant renouvellement (étudiants, post-doctorants…) Compétences linguistiques Le contexte international du laboratoire implique une compréhension écrite et orale de l’anglais

Expérience et contrat:

Bac +3, DUT ou BTS. Une expérience de 2 ou 3 ans en laboratoire de recherche est souhaitée. Il s’agit d’un CDD de 1 an éventuellement renouvelable. Les candidatures doivent être soumises à jean-rene.huynh@curie.fr et juliette.mathieu@curie.fr avec une lettre de motivation, un CV et les coordonnées de référents. Prise de poste : Septembre 2016.

Ingénieur d’étude ou Assistant Ingénieur

Affectation :          Institut Curie – Laboratoire de « Polarité Division et Morphogenèse » – Paris 5ème

Disponibilité :        Dès que possible

Contrat :                CDD 18 mois.

Le Centre de Recherche de l’Institut Curie (Paris, 5e) propose un emploi au sein de son laboratoire « Polarité Division Morphogenèse » dans le cadre du développement d’un projet de recherche visant à comprendre les mécanismes moléculaires régulant le développement des tissus épithéliaux.

Activités:

Au sein d’une équipe dynamique, votre mission sera

  • De réaliser des constructions ADN nécessaire à l’expression de gènes étiquetés par des protéines fluorescentes (GFP/mCherryFP).
  • De participer à la caractérisation des transcriptomes des tissus au cours du développement.

Profil Recherché :

– Bonne pratique oral et écrite de l’anglais.

– Une à 2 années d’expérience pratique en biologie moléculaire (clonage, PCR, transformation) sont absolument requises et/ou en analyse d’expression (RT-PCR, extraction et préparation ARNs pour séquençage à haut débit…)

– Connaissance des outils informatiques requis pour la recherche de séquences et leur manipulation.

être méthodique, organisé(e) et faire preuve d’une grande rigueur

Merci d’adresser CV, lettres de références et une lettre de motivation à yohanns.bellaiche@curie.fr

PROCESSING AND IMAGE ANALYSIS ENGINEER IN FLUORESCENCE MICROSCOPY

The Genetics and Development Biology Unit (http://www.ugbdd.curie.fr) of the Institut
Curie is a multidisciplinary department whose main objective is the study of events
controlling cell dynamics during development. The research teams intensively use
optical fluorescence microscopy to visualize in real time different biological processes
in various animal models (Drosophila, zebrafish and mouse).
Under the Labex-DEEP program (www.labex-deep.fr), we recruit a processing and
image analysis engineer to analyse large multidimensional microscopy data sets
acquired by researchers.

Missions
• Designing and implementing tools for image analysis and processing using ImageJ /
Fiji / Icy and Matlab.
• Set-up advanced workflow. e.g. image segmentation, quantify intracellular protein
distribution, pattern recognition, tracking of dynamic particle …
• Work with scientists for writing / implementing algorithms for solving image
processing problems from multidimensional datasets acquired using fluorescence
microscopy.
• Improve existing tools (computing speed, user interface, creating workflow).
• Validation of developed tools.
• Analysis of multidimensional microscopy datasets acquired by researchers.
• Train scientists in the use of developed tools and make tutorials.

Skills
• University degree (Master, PhD), engineering school or equivalent degree with a
strong specialization in image processing and analysis.
• Proven experience in the development of tools to process and analyse
multidimensional fluorescence microscopy datasets using ImageJ / Fiji / ICY (macros
and java plugin) and/or Matlab.
• Good knowledge of segmentation algorithms, object tracking, pattern recognition.
• Ability to work in teams and have an interest in multidisciplinary approaches in
developmental biology.
• Ability to manage multiple projects simultaneously. A high degree of autonomy is
required.
• Able to communicate in English.

Please send your CV, cover letter and contact information for two references
to: recrutement.labex.deep@curie.fr
Contract: COD (defined time contract) for 12 months (renewable to 36 months). Salary
depending on experience and qualifications.
Applications will be reviewed when they are received.

SGA Platform Technician or Trainee technician

Type of contract : CDD / full time – 4 months

Contract dates: 24/08/15 – 24/12/15

Context : After yeast genome sequencing in 1996 and the set up of deleted mutant collections in 2000, genomic techniques have developped tremendously. The Synthetic Genetic Array (SGA) is one of these high throughput emerging techniques, enabling the screening of double mutant banks. The principle is to cross a strain carrying a mutation in the gene of interest with a mutant bank. The double mutants generated are screened for their potential lethal synthetic gene interactions. This type of screening has been extensively used to decipher the complexity of genetic networks and pathways in yeast. Today, the SGA represents a powerful tool for studies of yeast gene functions. In addition to this technique, the SGA platform also offers optical density follow-up in 96-well plates (growth curve, sensitivity test,…). The SGA platform, part of the UMR3664 is located on the Paris Campus of Institut Curie.

Team website : http://umr3664.curie.fr/fr/plateforme/SGA

Description :

Tasks :

  • Take over the experimental part of the projects;
  • Work independantly under the supervision of the platform manager;
  • Manage and maintain the yeast strains and plasmids;
  • Ensure monitoring of the equipment and the proper functionning of the platform ;
  • Order and manage the consumables;
  • Report on the work progress and the results to the platform manager ;

Qualifications :

  • Level Bac +2/3 in microbiology
  • Good knowledge of microbiology and yeast genetics.
  • Expertise in various liquid and solid culture media.
  • Expertise in molecular biology classical techniques : mini-prep, PCR, cloning, …
  • Mastery of IT tools.
  • Mastery of English is required.

Skills :

  • Rigour, autonomy, dynamism
  • Good interpersonal skills

Contact : Please send a letter of motivation, a CV and your references by email to Petra KAFERLE and Manolis PAPAMICHOS-CHRONAKIS:

petra.kaferle@curie.fr

manolis.papamichos@curie.fr

 

Deadline for applications : July 24th 2015

Postdoctoral Position in Yeast Nuclear Organization

We are looking for a driven Postdoctoral Research Scientist with knowledge of the
fields of DNA repair and genome stability to join the « Compartmentalization and
Dynamics of nuclear Function » group headed by Dr Angela Taddei at Institut Curie, Paris.

Current work of the lab focuses on determining the spatial and temporal behaviour of chromatin and how this affects two essential functions of the genome: gene expression and the maintenance of genome integrity. To understand these fundamental processes, we are using yeast as a model system to which we can apply genetics, molecular biology and live advanced microscopy techniques.

The position is initialy funded by the FRM (« Fondation pour la Recherche Médicale ») and is fixed term for up to 2 years in the first instance with the possibility of an extension.

Qualifications
You will have a PhD in biological or biochemical sciences and demonstrable experience in molecular and cell biology techniques.
You will need strong organisational, teamworking and communication skills and a proven track record in taking projects from conception to completion. You will have a CV and publication record that would be competitive for international postdoctoral fellowships. Candidates with previous experience in yeast biology and/or advanced light microscopy will be favored.

Application details
Applications including a CV, names, contact details of two or three referees and a summary of current and future research interests should be submitted by e-mail to angela.taddei@curie.fr
Please indicate: « Postdoc application » in the subject line

2 Junior Group Leader positions

The Institut Curie research center is a world-class multidisciplinary cancer research center linked to a hospital. It combines research in biology, genetics, soft matter physics, organic and medicinal chemistry. The institute facilities include an advanced imaging platform with a wide variety of top-of-the line microscopes from confocal imaging to super-resolution, for cell and small animal imaging. Other facilies include a small molecule collection platform, high throughput sequencing, bioinformatics, reverse phase protein array, proteomics and mass spectrometry, antibody technologies and protein purification, nano-SIMS, cytometry, and animal housing (http://curie.fr/fr/recherche/technological-equipment-and-platforms). Access to unique clinical databases and sample collections enable active translational work and clinical applications.

As part of its strategic research axis Developmental Biology, Cancer, Genetics and Epigenetics, the Institut Curie is supporting the opening of two Junior Group Leader positions on its research campus in central Paris, in a vibrant international environment, to join respectively:

The Nuclear Dynamics department (http://umr3664.curie.fr/en) including epigenetics, genome functions, nuclear organization and chromosome biology.

Deadline for applications May 15th, 2015 to call.umr3664@curie.fr.

– The Genetics and Developmental Biology department (http://ugbdd.curie.fr/en) broadly interested in developmental and cell biology including organogenesis, morphogenesis, stem cells and physiology.

Deadline for applications June 30th 2015 to call.umr3215@curie.fr.

The newly recruited groups will benefit from the expertise of these units in epigenetics, chromatin, genetics, as well as developmental and cell biology and from state-of-the-art research equipment. Appropriate laboratory space for 6 persons and a start-up package will be available as well as support from the Labex DEEP (http://www.labex-deep.fr/) to which the two units belong. Successful candidates should meet criteria to obtain national and international funding, and for French institutional research positions (CNRS or Inserm).

Please send a personal statement explaining why you are interested in joining Institut Curie, a 3-4 page research plan, a full CV-detailing publications, patents, invited conferences, awards, grants, training and teaching experience- and contact details of 3-5 individuals who can be contacted for recommendation letters. Short listed candidates will be invited to present their research proposals and past achievements.

Deadlines for applications: May 15th 2015 / June 30th 2015

Click here to view poster of the call

Position: Research Engineer

Offer: 9 month contract (CDD), possibility for renewal

Place: Institut Curie Research Center – Paris 5th Arrondissement

Unit: CNRS UMR3664, Chromatin Dynamics team, directed by Dr. Genevieve Almouzni

Activities:

The selected candidate will join the chromatin dynamics team directed by Dr. Genevieve Almouzni (http://umr3664.curie.fr/fr/equipes/GA). This team is interested by epigenetic mechanisms and how they are implicated in regulating the organization of DNA within the cell nucleus during development and the cell cycle in normal and pathological situations.

The selected candidate will be an integral member of the team, involved in developing scientific projects within the scope of the laboratory with an expertise with mouse and xenopus model organisms.

Missions:

  • Develop a project with strong scientific and technological potential; validate and interpret results
  • Develop biochemistry to fractionate cellular components from various sources (i.e : xenopus eggs/ embryos extracts, various mammalian cells in culture, and mouse tissues & embryos)
  • Implement molecular and cellular biology and proteomics approaches
  • Apply new techniques enabling quantitative analyses and bioinformatics approaches.
  • Report on, scout and advise team members on new techniques and perform due diligence for the particular project, and participate in their setting up and guiding other users
  • Conduct literature searches to integrate the scientific project within the field and overcome any technical problems that may arise
  • Write progress reports and update lab protocols and technological resources
  • Stay current on evolving techniques and the field of genome organization and related areas and maintain proper records using IT tools (EndNote, Papers)

Required Experience:

  • 5 years of experience working in chromatin / genome organization (academia or industry)
  • Expertise in biochemistry, molecular biology of chromatin proteins (immunoprecipitation, preparation of nucleosomes, qPCR, ChIP…), and genome-wide analysis
  • Experience in working with whole organism experiments (mouse, xenopus…)
  • Expertise in mammalian cell culture (transfections, generation of stable cell lines)
  • Strong knowledge of developmental biology
  • Experience in microscopy (confocal, live-imaging) and image analysis (ImageJ, Adobe Photoshop)
  • Capacity to design, plan, and execute experiments independently
  • Master of office tools (Word, Powerpoint, Excel, Adobe Illustrator) and data management software (LIMS)
  • Ability toimplementthe logistic andscientific organizationfor the team
  • Sense of responsibility, communication, and team-spirit
  • Spoken and written English required.

Required training:

PhD in cellular or molecular biology or a related discipline and professional experience

 

Contact:

Send a CV, a cover letter, and 3 reference letters to isabelle.vassias@curie.fr