Colon tumours are hierarchically organized and contain multipotent self-renewing cells, called Cancer Stem Cells (CSCs). We have previously shown that the Notch1 receptor is expressed in Intestinal Stem Cells (ISCs); given the critical role played by Notch signalling in promoting intestinal tumourigenesis, we explored Notch1 expression in tumours. Combining lineage tracing in two tumour models with transcriptomic analyses, we found that Notch1+ tumour cells are undifferentiated, proliferative and capable of indefinite self-renewal and of generating a heterogeneous clonal progeny. Molecularly, the transcriptional signature of Notch1+ tumour cells highly correlates with ISCs, suggestive of their origin from normal crypt cells. Surprisingly, Notch1+ expression labels a subset of CSCs that shows reduced levels of Lgr5, a reported CSCs marker. The existence of distinct stem cell populations within intestinal tumours highlights the necessity of better understanding their hierarchy and behaviour, to identify the correct cellular targets for therap
Chromatin based control of germ cell and early embryonic development in mice
This course will explore the versatility of non-coding RNAs, a diverse and prevailing class of transcripts that engage in numerous biological processes across every branch of life in all living organisms from bacteria to humans. Word-famous researches will expose their very last discoveries in identification and biogenesis to functional roles of non-coding RNAs in physiology and human disease. It will provide an opportunity to young students and research fellows to discuss their work with an international scientific community in a warm and stimulating environment of Institut Curie.