Chromatin based control of germ cell and early embryonic development in mice
Mapping biochemical drivers of phenotypic change
Sabine Oertelt-Prigione, MD MSc PhD, is the strategic chair for gender in primary and transmural care at Radboud University in the Netherlands. Before this she worked at Charité-Universitätsmedizin in Berlin, Germany, at the University of California at Davis, USA, and at the University of Milan, Italy. She is specialized in internal medicine, public health and gender medicine and trained as an organizational consultant. She has published widely on aspects of sex and gender in medicine and gender-sensitive prevention. She is a frequent speaker at national and international conferences, member of numerous professional societies, expert counsel on the matter of sexual harassment prevention to German political institutions and pro-bono advisor for young scientists, non-profits and start-ups.
Integrating sex and gender into (bio)medical research
Chromatin and Transcription Dynamics in the Germline and Early Embryos
“SNAP-tag technology exposes centrosome and histone dynamics in mice”
- Daniel (Ming Kang) Lee (UMR3215, Raphaël Margueron’s team) – BAP1: the unexpected role of polycomb deubiquitinase in gene activation
- Justine Marsolier (UMR3244, Céline Vallot’s team) – Modeling epigenetic and transcriptomic plasticity in response and resistance to treatment in human Triple Negative Breast Cancer
- Raphaël Rodriguez (invited, UMR3666, Institut Curie Domain 4) – Reprogramming the reactivity of iron in cancer
Agenda to come.
Title & abstract TBD.
Title & abstract TBD.
Title & abstract TBD.
Agenda to come.
The single-cell symposium at Institut Curie will focus on multiple aspects of single-cell technologies applied to (epi)genomics, transcriptomics and proteomics, with 4 sessions dedicated to these aspects. This conference will provide opportunities to get up-to-date with the most recent single-cell data, generated to understand fundamental mechanisms of biology. All invited speakers have been selected worldwide for their scientific excellence in their field. This meeting will provide a unique forum to bring together a broad group of scientists with expertise in single-cell technologies and data analysis. We expect this meeting to encourage communication and promote networking between researchers involved in rapidly-developing emerging fields connected with single-cell technologies. There will be stimulating lectures on fascinating new topics, and new insights from already well-established lines of research. The environment of Institut Curie –next to international institutes such as ENS, College de France, Institut Pasteur and ESPCI– will attract numerous scientists that will exchange ideas around socializing events, sponsors’ stands and poster sessions.
Colour patterns are prominent features of most animals; they are highly variable and evolve rapidly leading to large diversities between species even within a single genus. As targets for natural as well as sexual selection, they are of high evolutionary significance. The zebrafish (Danio rerio) displays a conspicuous pattern of alternating blue and golden stripes on the body and on the anal- and tailfins. Pigment cells in zebrafish – melanophores, iridophores and xanthophores – originate from neural crest-derived stem cells associated with the dorsal root ganglia of the peripheral nervous system. Clonal analysis indicates that these progenitors remain multipotent and plastic beyond embryogenesis well into metamorphosis, when the adult colour pattern develops. Pigment cells share a lineage with neuronal cells of the peripheral nervous system; progenitors spread along the spinal nerves. The proliferation of pigment cells is regulated by competitive interactions among cells of the same type. An even spacing involves collective migration and contact inhibition of locomotion of the three cell types distributed in superimposed monolayers in the skin. This mode of colouring the skin is probably common to fish, whereas different patterns emerge by species specific cell interactions among the different pigment cell types. These interactions are mediated by channels involved in direct cell contact between the pigment cells, as well as unknown cues provided by the tissue environment.
The colour patterns in closely related Danio species are amazingly different; their variation offers a great opportunity to investigate the genetic and developmental basis of colour pattern evolution in vertebrates. Exciting technical developments of the recent years, especially next-generation sequencing technologies and the novel possibilities of genome editing with the CRISPR/Cas9 system, allow to easily expand from model organisms into other species and directly test the function of genes by targeted knock outs and allele replacements. Thus, models and hypotheses about pigment pattern formation derived from zebrafish can now be tested in other Danio species. These studies will lay the foundation to understand not only the genetic basis of colour pattern variation between Danio species, but also the evolution of colour patterns in other vertebrates.
Tiago Faial obtained his Ph.D. from the Stem Cell and Developmental Biology program at the University of Cambridge under the supervision of Jim Smith and Roger Pedersen, where he studied gene regulatory networks and signaling cascades that underpin mesoderm differentiation. For his postdoctoral work, Tiago joined Joanna Wysocka’s laboratory at Stanford University where he studied the dynamics of epigenetic landscapes in pluripotency. He joined the Nature Genetics editorial team in 2015.
Our genetic material (DNA) is continually subjected to damage, either from endogenous sources such as reactive oxygen species that arise as by-products of oxidative metabolism, from the breakdown of replication forks during cell growth, or by agents in the environment such as ionizing radiation or carcinogenic chemicals. To cope with such damage, cells employ a variety of repair processes that are specialized to recognize different types of lesions in DNA. These repair systems are essential for the maintenance of genome integrity and for cancer avoidance.
The focus of our research is to determine the mechanisms for repair and to define the cellular defects that lead to cancers and neurodegeneration, two common consequences of defective damage processing. In particular our efforts focus on the mechanisms of homologous recombination, which are important for the repair of double-strand breaks in DNA. Defects in this process lead to cancer predisposition, in particular breast cancers caused by mutation of the BRCA2 gene, acute leukemias associated with Fanconi anemia, and a wide range of cancers found in individuals with the chromosome instability disorder known as Bloom’s syndrome. Over the years, many of the proteins required for recombinational repair have been purified in our laboratory, and we use biochemical, structural, and molecular and cell biological approaches to understand how they bring about the repair of DNA breaks. Of particular interest are the roles of the BRCA2 tumour suppressor and the RAD51 recombinase in mediating the initiation of recombinational repair. Studies of the enzymes (MUS81-EME1 and GEN1) that mediate the resolution of DNA recombination intermediates (e.g. Holliday junctions) reveals an unexpectedly tightly controlled system that is essential not just for the completion of recombinational repair, but also for the proper segregation of DNA at mitosis. The lecture will describe our current understanding of recombinational repair and why defects in this process leads to human disease.
- 10-10h30: Julien Dumortier (Post-doc in Jean-Léon Maître’s team, UMR3215) Mechanics of lumen positioning in the mouse blastocyst: fracking and Ostwald ripening.
- 10:30-11h: Judith Mine Hattab (Researcher in Angela Taddei’s team, UMR3664) Gimme a break: Imaging Rad52 dynamics at the single molecule level.
- 11h-11h30: Michael Schertzer (Research Engineer inArturo Londono’s team, UMR3244) Nuclear envelope integrity and nucleo-cytoplasmic transport: implications for disease and aging.
- 11h30-12h: Annabelle Gerard (Director of External Innovation Partnership, HiFiBio Therapeutics) Single Cell Technologies Development : how HiFiBiO aims at accelerating drug discovery by leveraging innovation partnerships.